LEGE-CC

The Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC) is a vital resource for research, innovation, and education in both scientific and industrial fields. Hosted at CIIMAR in Matosinhos, Portugal, LEGE-CC was established in 1991 and is the oldest Portuguese culture collection dedicated to cyanobacteria and microalgae. With over 1100 strains, LEGE-CC supports sustainable solutions for global challenges, from biomedical research to eco-friendly innovations. The collection includes isolates mainly from Portuguese ecosystems, including the Azores and Madeira, as well as isolated strains from international regions like Australia, Brazil, Colombia, Morocco, Mexico, Dominican Republic, Cape Verde and more. With its rich taxonomic and genetic diversity, LEGE-CC offers unmatched potential for discovery in fields where sustainable, nature-based solutions are increasingly in demand.

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What we Do

LEGE-CC is affiliated with networks like WFCC, ECCO, EMBRC, and MIRRI and plays a strategic role in advancing blue biotechnology. Its strains have contributed to studies on cancer, antibiotics, anti-obesity treatments, biofouling prevention, cosmetics and food. Several studies demonstrated that our LEGE-CC strains have potential and effective capacity to produce a diverse array of metabolites, including toxins (e.g. microcystins, anatoxin, cylindrospermopsin and BMAA) or newly discovered molecules (Portoamides, Hierridin B and C, Bartolosides, Nocuolin A, etc). As other biological resource centers worldwide, LEGE-CC seeks to provide several services (e.g. provision of starter cultures for diverse purposes, isolation and identification of strains, cyanotoxins analysis, cryopreservation, training courses, etc.).

Our Services

Strain Identification

LEGE-CC offers identification services for cyanobacterial and microalgae strains not included in the culture collection, available upon external request. The process follows modern taxonomic standards, combining molecular (16S or 18S and morphological analyses, and results in a comprehensive identification report.

Culture Supply

LEGE-CC provides living Cyanobacteria and microalgae cultures, in liquid medium, supplied in two 5 ml tubes per order. Shipping is scheduled 3 to 4 weeks following confirmation of order and payment.

Strain Deposit

LEGE-CC provides a deposition and maintenance service for cyanobacterial and microalgae strains. [Price list] LEGE-CC staff will verify their identity and purity of the strains. If the strain passes this initial assessment, it will be handled as stated in the Private Collection Deposit Form for the stated term of the agreement. Please refer to the Deposit Form in the Knowledgebase for full guidelines. For inquiries or to start the process, contact us at legeculturecollection@ciimar.up.pt. Validated strains are assigned a unique LEGE-CC code. Strains are maintained in active liquid culture, and will grow under standard LEGE-CC conditions.

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